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Article

Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation.

PloS one - 1 Jan 2014

Tang Guo-Qing, Nandakumar Divya, Bandwar Rajiv P, Lee Kyung Suk, Roy Rahul, Ha Taekjip, Patel Smita S

Abstract excerpt

Abortive cycling is a universal feature of transcription initiation catalyzed by DNA-dependent RNA polymerases (RNAP). In bacteriophage T7 RNAP, mutation of proline 266 to leucine (P266L) in the C-linker region connecting the N-terminal promoter binding domain with the C-terminal catalytic domain drastically reduces short abortive products (4-7 nt) while marginally increasing long abortives (9-11 nt). Here we...

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Identifiers

PMID: 24651161
DOI: 10.1371/journal.pone.0091859
PMCID: PMC3961267

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Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation.PMID 24651161Read ↗

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase.Semantic proximity Read ↗

Sequential release of promoter contacts during transcription initiation to elongation transition.Semantic proximity Read ↗

A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription.Semantic proximity Read ↗

Cross-linking of promoter DNA to T7 RNA polymerase does not prevent formation of a stable elongation complex.Semantic proximity Read ↗

The transition to an elongation complex by T7 RNA polymerase is a multistep process.Semantic proximity Read ↗

Regulation of promoter-proximal transcription elongation: enhanced DNA scrunching drives λQ antiterminator-dependent escape from a σ70-dependent pause.Semantic proximity Read ↗

An alternate mechanism of abortive release marked by the formation of very long abortive transcripts.Semantic proximity Read ↗

Functional architecture of T7 RNA polymerase transcription complexes.Semantic proximity Read ↗

Open complex scrunching before nucleotide addition accounts for the unusual transcription start site of E. coli ribosomal RNA promoters.Semantic proximity Read ↗

Characterization of a set of T7 RNA polymerase active site mutants.Semantic proximity Read ↗

Engineering of T7 DNA-dependent RNA polymerase with activity at elevated temperature.Semantic proximity Read ↗

The intercalating beta-hairpin of T7 RNA polymerase plays a role in promoter DNA melting and in stabilizing the melted DNA for efficient RNA synthesis.Semantic proximity Read ↗

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