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Article

Cross-linking of promoter DNA to T7 RNA polymerase does not prevent formation of a stable elongation complex.

The Journal of biological chemistry - 22 Oct 2004

Esposito Edward A, Martin Craig T

Abstract excerpt

T7 RNA polymerase recognizes a small promoter, binds DNA, and begins the process of transcription by synthesizing short RNA products without releasing promoter contacts. To determine whether the promoter contact must be released to make longer RNA products and at what position the promoter must be released, a mutant RNA polymerase was designed that allows cross-linking to a modified promoter via a covalent...

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Identifiers

PMID: 15304497
DOI: 10.1074/jbc.M407688200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Cross-linking of promoter DNA to T7 RNA polymerase does not prevent formation of a stable elongation complex.PMID 15304497Read ↗

Weakening of the T7 promoter-polymerase interaction facilitates promoter release.Semantic proximity Read ↗

A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription.Semantic proximity Read ↗

Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase.Semantic proximity Read ↗

Sequential release of promoter contacts during transcription initiation to elongation transition.Semantic proximity Read ↗

Probing conformational changes in T7 RNA polymerase during initiation and termination by using engineered disulfide linkages.Semantic proximity Read ↗

Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation.Semantic proximity Read ↗

T7 RNA polymerases backed up by covalently trapped proteins catalyze highly error prone transcription.Semantic proximity Read ↗

Functional architecture of T7 RNA polymerase transcription complexes.Semantic proximity Read ↗

Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability.Semantic proximity Read ↗

A mutant RNA polymerase that forms unusual open promoter complexes.Semantic proximity Read ↗

Open complex scrunching before nucleotide addition accounts for the unusual transcription start site of E. coli ribosomal RNA promoters.Semantic proximity Read ↗

Dissociation of halted T7 RNA polymerase elongation complexes proceeds via a forward-translocation mechanism.Semantic proximity Read ↗

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