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Article

The thumb subdomain of T7 RNA polymerase functions to stabilize the ternary complex during processive transcription.

The Journal of biological chemistry - 7 Oct 1994

Bonner G, Lafer E M, Sousa R

Abstract excerpt

To examine the function of the thumb subdomain in bacteriophage T7 RNA polymerase we constructed a set of deletion mutants within this subdomain. These mutants exhibited reduced processivity during the processive, but not the abortive, stage of transcription. Reduced processivity was found to be due primarily to an increase in the processive ternary complex dissociation rate (destabilization of the processive...

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PMID: 7523379

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