Article
Characterization of a Mycobacterium tuberculosis H37Rv transposon library reveals insertions in 351 ORFs and mutants with altered virulence.
Microbiology (Reading, England) - 1 Oct 2002
McAdam Ruth A, Quan Selwyn, Smith Debbie A, Bardarov Stoyan, Betts Joanna C, Cook Fiona C, Hooker Elizabeth U, Lewis Alan P, Woollard Peter, Everett Martin J, Lukey Pauline T, Bancroft Gregory J, Jacobs William R, Duncan Ken
Abstract excerpt
A library of Mycobacterium tuberculosis insertional mutants was generated with the transposon Tn5370. The junction sequence between the transposon and the mycobacterial chromosome was determined, revealing the positions of 1329 unique insertions, 1189 of which were located in 351 different ORFs. Transposition was not completely random and examination of the most susceptible genome regions revealed a...
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