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Article

Identifying essential genes in Mycobacterium tuberculosis by global phenotypic profiling.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Long Jarukit E, DeJesus Michael, Ward Doyle, Baker Richard E, Ioerger Thomas, Sassetti Christopher M

Abstract excerpt

Transposon sequencing (TnSeq) is a next-generation deep sequencing-based method to quantitatively assess the composition of complex mutant transposon libraries after pressure from selection. Although this method can be used for any organism in which transposon mutagenesis is possible, this chapter describes its use in Mycobacterium tuberculosis. More specifically, the methods for generating complex libraries...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 25636614
DOI: 10.1007/978-1-4939-2398-4_6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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