Article
Alternative secondary structures in the 5' exon affect both forward and reverse self-splicing of the Tetrahymena intervening sequence RNA.
Biochemistry - 26 Feb 1991
Woodson S A, Cech T R
Abstract excerpt
The natural splice junction of the Tetrahymena large ribosomal RNA is flanked by hairpins that are phylogenetically conserved. The stem immediately preceding the splice junction involves nucleotides that also base pair with the internal guide sequence of the intervening sequence during splicing. Thus, precursors which contain wild-type exons can form two alternative helices. We have constructed a series of RNAs...
Topics
- Animals
- Base Composition
- Base Sequence
- Exons
- Genetic Variation
- Introns
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Plasmids