Article
Fitness landscape of a dynamic RNA structure.
PLoS genetics - 1 Feb 2021
Soo Valerie W C, Swadling Jacob B, Faure Andre J, Warnecke Tobias
Abstract excerpt
RNA structures are dynamic. As a consequence, mutational effects can be hard to rationalize with reference to a single static native structure. We reasoned that deep mutational scanning experiments, which couple molecular function to fitness, should capture mutational effects across multiple conformational states simultaneously. Here, we provide a proof-of-principle that this is indeed the case, using the...
Topics
- Base Sequence
- Evolution, Molecular
- Genetic Fitness
- Genetic Pleiotropy
- Genotype
- Introns
- Kinetics
- Machine Learning
- Mutation
- Nucleic Acid Conformation
- RNA
- RNA Splice Sites
- RNA Splicing
- RNA, Protozoan
- Tetrahymena thermophila