Article
Construction of an expression system of insect lysozyme lacking thermal stability: the effect of selection of signal sequence on level of expression in the Pichia pastoris expression system.
Protein engineering - 1 Sept 2001
Koganesawa N, Aizawa T, Masaki K, Matsuura A, Nimori T, Bando H, Kawano K, Nitta K
Abstract excerpt
Expression systems of human and silkworm lysozymes were constructed using the methylotrophic yeast Pichia pastoris as a host. The leader sequence and its prepro peptide of alpha-factor (a peptide pheromone derived from yeast) and the native signal sequences of these lysozymes, were used as secretion signals. When the alpha-factor leader is used as the signal sequence, human lysozyme is secreted at a much higher...
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