Article
Effect of N-terminal mutation of human lysozyme on enzymatic activity.
Nucleic acids symposium series (2004) - 1 Jan 2007
Tsuchiya Yoshinori, Morioka Kazuki, Yoshida Kazuo, Shirai Junsuke, Kokuho Takehiro, Inumaru Shigeki
Abstract excerpt
Using mutant signal peptide introduced Pro at the C-terminal region, 5 kinds of N-terminal mutants of human lysozyme (HLY) were produced. The genes coding chicken lysozyme signal peptide - human lysozyme (HLY) hybrid preproteins were altered as follows: -2Leu to Pro and -lGly to either Ala, Val, Leu, Asn or Lys, and were expressed in yeast cells. The N-terminal sequence data of HLYs secreted from yeast cells,...
Topics
- Humans
- Hydrogen-Ion Concentration
- Muramidase
- Mutation
- Osmolar Concentration
- Protein Precursors
- Protein Sorting Signals