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The impact of ERAD on recombinant protein secretion in Pichia pastoris (syn Komagataella spp.).

Microbiology (Reading, England) - 1 Apr 2018

Zahrl Richard J, Mattanovich Diethard, Gasser Brigitte

Abstract excerpt

The yeast Pichia pastoris (syn. Komagataella spp.) is a popular cell factory for recombinant protein production. Yeasts in general provide a good starting point for cell factory engineering. They are intrinsically robust and easy to manipulate and cultivate. However, their secretory pathway is not evolutionarily adapted to high loads of secretory protein. In particular, more complex proteins, like the antibody...

Topics

Endoplasmic Reticulum-Associated Degradation
Fungal Proteins
Immunoglobulin Fab Fragments
Metabolic Engineering
Mutation
Pichia
Protein Folding
Protein Transport
Recombinant Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 29533745
DOI: 10.1099/mic.0.000630

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