The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris.

doi:10.1016/j.gene.2013.01.062

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Article

The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris.

Gene - 1 May 2013

Lin-Cereghino Geoff P, Stark Carolyn M, Kim Daniel, Chang Jennifer, Shaheen Nadia, Poerwanto Hansel, Agari Kimiko, Moua Pachai, Low Lauren K, Tran Namphuong, Huang Amy D, Nattestad Maria, Oshiro Kristin T, Chang John William, Chavan Archana, Tsai Jerry W, Lin-Cereghino Joan

Abstract excerpt

The methylotrophic yeast, Pichia pastoris, has been genetically engineered to produce many heterologous proteins for industrial and research purposes. In order to secrete proteins for easier purification from the extracellular medium, the coding sequence of recombinant proteins is initially fused to the Saccharomyces cerevisiae α-mating factor secretion signal leader. Extensive site-directed mutagenesis of the...

Topics

Amino Acid Sequence
Blotting, Western
Gene Deletion
Gene Expression Regulation, Fungal
Genes, Reporter
Horseradish Peroxidase
Lipase
Mating Factor
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Peptides

Pichia

Plasmids

Real-Time Polymerase Chain Reaction

Recombinant Proteins

Saccharomyces cerevisiae

Source

Open in PubMed Open full text

Identifiers

PMID: 23454485
DOI: 10.1016/j.gene.2013.01.062
PMCID: PMC3628533

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-23454485-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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