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Expression, purification, and characterization of an unstable lysozyme mutant in Pichia pastoris.

Protein expression and purification - 1 Feb 2003

Liu Shu-Tao, Saito Akira, Azakami Hiroyuki, Kato Akio

Abstract excerpt

To investigate the expression and purification of an unstable heterologous protein in Pichia pastoris, the cDNA of H5-lysozyme, a hen egg lysozyme mutant with a hydrophobic pentapeptide (Phe-Phe-Val-Ala-Pro) fused to the carboxyl terminus, was integrated into the genome of P. pastoris. It was found that medium composition, induction time, and fermenter type were important factors for the expression of...

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PMID: 12597890
DOI: 10.1016/s1046-5928(02)00642-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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