Article
Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris.
Microbial biotechnology - 1 Sept 2022
Ito Yoichiro, Ishigami Misa, Hashiba Noriko, Nakamura Yasuyuki, Terai Goro, Hasunuma Tomohisa, Ishii Jun, Kondo Akihiko
Abstract excerpt
In our previous study, we serendipitously discovered that protein secretion in the methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor alpha (MFα) prepro-leader signal derived from Saccharomyces cerevisiae. In the present study, we investigated 20 single-ami...
Topics
- Amino Acid Sequence
- Fungal Proteins
- Mating Factor
- Mutation
- Pichia
- Protein Sorting Signals
- Proteolysis
- Recombinant Proteins
- Saccharomyces cerevisiae
- Saccharomycetales