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Article

Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris.

Microbial biotechnology - 1 Sept 2022

Ito Yoichiro, Ishigami Misa, Hashiba Noriko, Nakamura Yasuyuki, Terai Goro, Hasunuma Tomohisa, Ishii Jun, Kondo Akihiko

Abstract excerpt

In our previous study, we serendipitously discovered that protein secretion in the methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor alpha (MFα) prepro-leader signal derived from Saccharomyces cerevisiae. In the present study, we investigated 20 single-amino-acid substitutions, including V50A, located within the MFα signal peptide, indicating that V50A and several single...

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Identifiers

PMID: 35656803
DOI: 10.1111/1751-7915.14061
PMCID: PMC9437885

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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