The molecular modification, expression, and the antibacterial effects studies of human lysozyme.

Abstract excerpt

Human lysozyme (hLYZ) has attracted considerable research attention due to its natural and efficient antibacterial abilities and widespread uses. In this study, hLYZ was modified to enhance its enzyme activity and expressed in a Pichia pastoris expression system. A combination mutant HZM(2R-K)-N88D/V110S demonstrated the highest enzyme activity (6213 ± 164 U/mL) in shake flasks, which was 4.07-fold higher when...

Topics

• Muramidase
• Anti-Bacterial Agents
• Humans
• Recombinant Proteins
• Staphylococcus aureus
• Bioreactors
• Micrococcus
• Gene Expression
• Mutation
• Saccharomycetales
• Microbial Sensitivity Tests