Article
Effect of chicken lysozyme signal peptide alterations on secretion of human lysozyme in Saccharomyces cerevisiae.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Tsuchiya Y, Fujisawa H, Nakayama K, Nagahora H, Jigami Y
Abstract excerpt
To investigate the structural requirements and functions of the N-terminal and the C-terminal regions of the chicken lysozyme signal peptide, the amino acids of each region were altered. The replacement of Gly(-1) and Leu(-2) with Pro(-1) and Ala(-2) or Val(-2), respectively, resulted in the complete shift of the cleavage site from position -1 to -2 in yeast (Saccharomyces cerevisiae). This shows that the...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Chickens
- Gene Transfer Techniques
- Humans
- Molecular Sequence Data
- Muramidase
- Mutation
- Plasmids
- Protein Sorting Signals