Article
Development of a mouse cell line containing the PhiX174 am3 allele as a target for detecting mutation.
Mutation research - 18 Aug 1999
Chen J B, Dobrovolsky V N, Heflich R H
Abstract excerpt
Transgenic mice containing multiple copies of the PhiX174 am3 allele are being developed as a model for detecting tissue-specific in vivo mutation. In order to derive an analogous system for measuring am3 mutation in vitro, cells were cultured from 15-day-old C57Bl/6J mouse embryos that were homozygous for the transgene and these cells were transfected with a plasmid expressing the SV40 large T-antigen. Two...
Topics
- Alleles
- Animals
- Bacteriophage phi X 174
- Cell Line
- DNA Damage
- Ethylnitrosourea
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Mutation