Article
Development of a short-term, in vivo mutagenesis assay: the effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice.
Nucleic acids research - 25 May 1990
Kohler S W, Provost G S, Kretz P L, Dycaico M J, Sorge J A, Short J M
Abstract excerpt
Transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. The shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic DNA with in vitro packaging extracts. Mutations in the target gene are detected by a change in lambda phage plaque color on indicator...
Topics
- Animals
- Azacitidine
- Bacteriophage lambda
- Blotting, Southern
- Cells, Cultured
- DNA
- Escherichia coli
- Ethylnitrosourea
- Fibroblasts
- Genetic Vectors
- Methylation