Article
A base change in the catalytic core of the hairpin ribozyme perturbs function but not domain docking.
Biochemistry - 27 Feb 2001
Walter N G, Chan P A, Hampel K J, Millar D P, Burke J M
Abstract excerpt
The hairpin ribozyme is a small endonucleolytic RNA motif with potential for targeted RNA inactivation. It optimally cleaves substrates containing the sequence 5'-GU-3' immediately 5' of G. Previously, we have shown that tertiary structure docking of its two domains is an essential step in the reaction pathway of the hairpin ribozyme. Here we show, combining biochemical and fluorescence structure and function...
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