Article
Functional involvement of G8 in the hairpin ribozyme cleavage mechanism.
The EMBO journal - 15 Nov 2001
Pinard R, Hampel K J, Heckman J E, Lambert D, Chan P A, Major F, Burke J M
Abstract excerpt
The catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are integral to the polyribonucleotide chain. We describe experiments that place G8, a critical guanosine, at the active site, and point to an essential role in catalysis. Cross-linking and modeling show that formation of a catalytic complex is accompanied by a conformational change in which N1 and O6 of G8 become...
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