Article
Three critical hydrogen bonds determine the catalytic activity of the Diels-Alderase ribozyme.
Nucleic acids research - 1 Feb 2012
Kraut Stefanie, Bebenroth Dirk, Nierth Alexander, Kobitski Andrei Y, Nienhaus G Ulrich, Jäschke Andres
Abstract excerpt
Compared to protein enzymes, our knowledge about how RNA accelerates chemical reactions is rather limited. The crystal structures of a ribozyme that catalyzes Diels-Alder reactions suggest a rich tertiary architecture responsible for catalysis. In this study, we systematically probe the relevance of crystallographically observed ground-state interactions for catalytic function using atomic mutagenesis in...
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