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Article

Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability.

Journal of molecular biology - 6 Oct 2000

Mentesana P E, Chin-Bow S T, Sousa R, McAllister W T

Abstract excerpt

We have constructed a series of plasmid templates that allow T7 RNA polymerase (RNAP) to be halted at defined intervals downstream from its promoter in a preserved sequence context. While transcription complexes halted at +3 to +6 are highly unstable, complexes halted at +10 to +14 dissociate very slowly and gradually lose their capacity to extend transcripts. Complexes halted at +18 and beyond dissociate more...

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Identifiers

PMID: 11183774
DOI: 10.1006/jmbi.2000.4114

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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