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Article

Probing conformational changes in T7 RNA polymerase during initiation and termination by using engineered disulfide linkages.

Proceedings of the National Academy of Sciences of the United States of America - 6 Dec 2005

Ma Kaiyu, Temiakov Dmitry, Anikin Michael, McAllister William T

Abstract excerpt

During the transition from an initiation complex to an elongation complex (EC), the single-subunit bacteriophage T7 RNA polymerase (RNAP) undergoes dramatic conformational changes. To explore the significance of these changes, we constructed mutant RNAPs that are able to form disulfide bonds that limit the mobility of elements that are involved in the transition (or its reversal) and examined the effects of the...

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Identifiers

PMID: 16301518
DOI: 10.1073/pnas.0508865102
PMCID: PMC1308916

Genes

PTH

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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