Article
RNA chain elongation by Escherichia coli RNA polymerase. Factors affecting the stability of elongating ternary complexes.
Journal of molecular biology - 5 May 1990
Arndt K M, Chamberlin M J
Abstract excerpt
We have devised a method to follow the stability of individual ternary transcription complexes containing Escherichia coli RNA polymerase halted at many different sites along a DNA template during the transcription process. Studies of complexes formed with phage T7 DNA templates reveal at least three general classes of ternary complexes that differ dramatically in their properties. Complexes of one sort (normal...
Topics
- Base Sequence
- Chromatography, Gel
- DNA, Viral
- DNA-Directed RNA Polymerases
- Escherichia coli
- Half-Life
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligodeoxyribonucleotides
- RNA
