Article
Analysis of the accumulation of mutants in Sabin attenuated polio vaccine viruses passaged in Vero cells.
Vaccine - 8 Jan 2001
Horie H, Miyazawa M, Ota Y, Wakabayashi K, Yoshida H, Doi Y, Hashizume S
Abstract excerpt
To confirm the safety of oral poliomyelitis vaccine (OPV) cultured in Vero cells, the genetic stability of cultured polio vaccine viruses was analysed by MAPREC (mutant analysis by PCR and restriction enzyme cleavage). The rates of mutant accumulation of the viruses passaged in Vero cells under a low multiplicity of infection (MOI) condition (approximately 10(-3.5)CCID50/cell; the same as under usual OPV...
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