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Article

Massively parallel sequencing for monitoring genetic consistency and quality control of live viral vaccines.

Proceedings of the National Academy of Sciences of the United States of America - 16 Nov 2010

Neverov Alexander, Chumakov Konstantin

Abstract excerpt

Intrinsic genetic instability of RNA viruses may lead to the accumulation of revertants during manufacture of live viral vaccines, requiring rigorous quality control to ensure vaccine safety. Each lot of oral poliovirus vaccine (OPV) is tested for neurovirulence in animals and also for the presence of neurovirulent revertants. Mutant analysis by PCR and restriction enzyme cleavage (MAPREC) is used to measure the...

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Identifiers

PMID: 21041640
DOI: 10.1073/pnas.1012537107
PMCID: PMC2993378

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