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Article

Allele-specific PCR for quantitative analysis of mutants in live viral vaccines.

Journal of virological methods - 1 Jun 2014

Bidzhieva Bella, Laassri Majid, Chumakov Konstantin

Abstract excerpt

Monitoring consistency of genetic composition of oral polio vaccine (OPV) is a part of its quality control. It is performed by mutant analysis by PCR and restriction enzyme cleavage (MAPREC) used to quantify neurovirulent revertants in the viral genome. Here an alternative method based on quantitative PCR is proposed. Allele-specific quantitative polymerase chain reaction (asqPCR) uses a "tethered"...

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Identifiers

PMID: 24607431
DOI: 10.1016/j.jviromet.2014.02.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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