Article
Consistent selection of mutations in the 5'-untranslated region of oral poliovirus vaccine upon passaging in vitro.
Journal of medical virology - 1 Jan 1994
Chumakov K M, Dragunsky E M, Norwood L P, Douthitt M P, Ran Y, Taffs R E, Ridge J, Levenbook I S
Abstract excerpt
We have previously found that upon passaging type 3 oral poliovirus vaccine (OPV) in cell cultures the proportion of revertants at nucleotide 472 rapidly increases [Chumakov et al.: Proceedings of the National Academy of Sciences of the United States of America 88:199-203 1991]. Systematic study on the accumulation of these revertants showed that it was dependent on the multiplicity of infection and the...
Topics
- Base Sequence
- Cell Line
- DNA Mutational Analysis
- DNA Primers
- DNA, Complementary
- Genes, Viral
- Molecular Sequence Data
- Mutation
- Poliovirus
- Poliovirus Vaccine, Oral
- RNA, Viral