Article
Microevolution of Sabin 1 strain in vitro and genetic stability of oral poliovirus vaccine.
Virology - 1 Jul 1994
Rezapkin G V, Chumakov K M, Lu Z, Ran Y, Dragunsky E M, Levenbook I S
Abstract excerpt
Mutants consistently accumulating in Sabin 1 poliovirus during serial passaging in vitro were identified by sequence heterogeneity assay and quantitated using mutant analysis by PCR and restriction enzyme cleavage (MAPREC). Only four unstable genomic sites were identified in virus passaged 10 times in African green monkey kidney (AGMK) cells, and eight sites in virus passaged in Vero cells. Mutations accumulated...
Topics
- Animals
- Base Sequence
- Biological Evolution
- Cell Line
- Chlorocebus aethiops
- DNA Mutational Analysis
- DNA, Viral
- Genetic Variation
- Introns
- Macaca mulatta
- Molecular Sequence Data