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Article

Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains.

Nucleic acids research - 1 Sept 2000

Smith J, Bibikova M, Whitby F G, Reddy A R, Chandrasegaran S, Carroll D

Abstract excerpt

This study concerns chimeric restriction enzymes that are hybrids between a zinc finger DNA-binding domain and the non-specific DNA-cleavage domain from the natural restriction enzyme FOK:I. Because of the flexibility of DNA recognition by zinc fingers, these enzymes are potential tools for cleaving DNA at arbitrarily selected sequences. Efficient double-strand cleavage by the chimeric nucleases requires two...

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Identifiers

PMID: 10954606
DOI: 10.1093/nar/28.17.3361
PMCID: PMC110700

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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