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Article

Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases.

Journal of molecular biology - 2 Jul 2010

Guo Jing, Gaj Thomas, Barbas Carlos F

Abstract excerpt

Zinc finger nucleases (ZFNs) are powerful tools for gene therapy and genetic engineering. The high specificity and affinity of these chimeric enzymes are based on custom-designed zinc finger proteins (ZFPs). To improve the performance of existing ZFN technology, we developed an in vivo evolution-...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 20447404
DOI: 10.1016/j.jmb.2010.04.060
PMCID: PMC2885538

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20447404-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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