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In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites

14 Aug 2013

Abstract excerpt

Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce mutations at off-target sites of action. Such unintended alterations can confound interpretation of experiments and can have implications for development of therapeutic applications. Recently,...

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PMID: 23945932
DOI: 10.1093/nar/gkt716

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