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Article

Stimulation of homologous recombination through targeted cleavage by chimeric nucleases.

Molecular and cellular biology - 1 Jan 2001

Bibikova M, Carroll D, Segal D J, Trautman J K, Smith J, Kim Y G, Chandrasegaran S

Abstract excerpt

Chimeric nucleases that are hybrids between a nonspecific DNA cleavage domain and a zinc finger DNA recognition domain were tested for their ability to find and cleave their target sites in living cells. Both engineered DNA substrates and the nucleases were injected into Xenopus laevis oocyte nuclei, in which DNA cleavage and subsequent homologous recombination were observed. Specific cleavage required two...

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Source

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Identifiers

PMID: 11113203
DOI: 10.1128/MCB.21.1.289-297.2001
PMCID: PMC88802

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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