Identification of a U2/U6 helix la mutant that influences 3' splice site selection during nuclear pre-mRNA splicing.

Abstract excerpt

Base substitutions in U2/U6 helix I, a conserved base-pairing interaction between the U6 and U2 snRNAs, have previously been found to specifically block the second catalytic step of nuclear pre-mRNA splicing. To further assess the role of U2/U6 helix I in the second catalytic step, we have screened mutations in U2/U6 helix I to identify those that influence 3' splice site selection using a derivative of the yeast...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Actins
• Share to Adenosine
• Share to Catalysis