Article
Mutations in U6 snRNA that alter splice site specificity: implications for the active site.
Science (New York, N.Y.) - 24 Dec 1993
Lesser C F, Guthrie C
Abstract excerpt
What determines the precise sites of cleavage in the two transesterification reactions of messenger RNA (mRNA) splicing is a major unsolved question. Mutation of the invariant G (guanosine) at position 5 of 5' splice sites in Saccharomyces cerevisiae introns activates cleavage at nearby aberrant...
Topics
- Base Sequence
- Binding Sites
- Genes, Reporter
- Introns
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Splicing
- RNA, Small Nuclear
- Saccharomyces cerevisiae
- Suppression, Genetic