Article
Randomization-selection analysis of snRNAs in vivo: evidence for a tertiary interaction in the spliceosome.
Genes & development - 1 May 1994
Madhani H D, Guthrie C
Abstract excerpt
Putative components of the spliceosomal active site include a bulged helix between U2 and U6 snRNAs (U2-U6 helix I) and the adjacent ACAGAG hexanucleotide in U6. We have developed an in vivo, bimolecular randomization-selection method to functionally dissect these elements. Although a portion of...
Topics
- Alleles
- Base Composition
- Base Sequence
- Binding Sites
- Gene Library
- Genetic Variation
- Introns
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Oligoribonucleotides
- Phenotype
- RNA Splicing
- RNA, Fungal
- RNA, Small Nuclear
- Saccharomyces cerevisiae
- Spliceosomes