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Article

Viral evolution as a tool to improve the tetracycline-regulated gene expression system.

The Journal of biological chemistry - 30 Apr 2004

Das Atze T, Zhou Xue, Vink Monique, Klaver Bep, Verhoef Koen, Marzio Giuseppe, Berkhout Ben

Abstract excerpt

We present viral evolution as a novel and powerful method to optimize non-viral proteins. We used this approach to optimize the tetracycline (Tc)-regulated gene expression system (Tet system) for its function in mammalian cells. The components of the Tet system were incorporated in the human immunodeficiency virus (HIV)-1 virus such that viral replication is controlled by this regulatory system. Upon long term...

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Identifiers

PMID: 14761948
DOI: 10.1074/jbc.M313895200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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