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Article

Positive and negative mutant selection in the human histone hairpin-binding protein using the yeast three-hybrid system.

Nucleic acids research - 1 Apr 2000

Martin F, Michel F, Zenklusen D, Müller B, Schümperli D

Abstract excerpt

We have used the yeast three-hybrid system in a positive selection for mutants of the human histone hairpin-binding protein (HBP) capable of interacting with non-canonical hairpins and in a negative selection for loss-of-binding mutants. Interestingly, all mutations from the positive selection are located in the N- and C-terminal regions flanking a minimal RNA-binding domain (RBD) previously defined between amino...

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Identifiers

PMID: 10710426
DOI: 10.1093/nar/28.7.1594
PMCID: PMC102788

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