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Article

In vitro selection of second site revertants analysis of the hairpin ribozyme active site.

The Journal of biological chemistry - 26 Dec 2003

Sargueil Bruno, Hampel Ken J, Lambert Dominic, Burke John M

Abstract excerpt

We have used in vitro genetics to evaluate the function and interactions of the conserved base G8 in the hairpin ribozyme catalytic RNA. Second site revertant selection for a G8X mutant, where X is any of the other three natural nucleobases, yielded a family of second site suppressors of the G8U mutant, but not of G8C or G8A, indicating that only G and U can be tolerated at position 8 of the ribozyme. This result...

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Identifiers

PMID: 14555657
DOI: 10.1074/jbc.M306703200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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