Article
Analysis of RNA-binding proteins by in vitro genetic selection: identification of an amino acid residue important for locking U1A onto its RNA target.
Proceedings of the National Academy of Sciences of the United States of America - 5 Dec 1995
Laird-Offringa I A, Belasco J G
Abstract excerpt
An in vitro genetic system was developed as a rapid means for studying the specificity determinants of RNA-binding proteins. This system was used to investigate the origin of the RNA-binding specificity of the mammalian spliceosomal protein U1A. The U1A domain responsible for binding to U1 small...
Topics
- Amino Acid Sequence
- Bacteriophages
- Base Sequence
- Binding Sites
- DNA, Recombinant
- Gene Library
- Genetic Variation
- Genetic Vectors
- Molecular Sequence Data
- Nucleic Acid Conformation
- RNA, Small Nuclear
- RNA-Binding Proteins
- Recombinant Proteins
- Ribonucleoprotein, U1 Small Nuclear
- Selection, Genetic
- Structure-Activity Relationship