Article
Validation of the human T-lymphocyte cloning assay--ring test report from the EU concerted action on HPRT mutation (EUCAHM).
Mutation research - 17 Dec 1999
Hou S M, Van Dam F J, de Zwart F, Warnock C, Mognato M, Turner J, Podlutskaja N, Podlutsky A, Becker R, Barnett Y, Barnett C R, Celotti L, Davies M, Hüttner E, Lambert B, Tates A D
Abstract excerpt
The T-cell cloning assay, which enables the enumeration and molecular analysis of 6-thioguanine resistant (HPRT-negative) mutant T-cells, has been extensively used for studying human somatic gene mutation in vivo. However, large inter-laboratory variations in the HPRT mutant frequency (MF) call for further investigation of inter-laboratory differences in the experimental methodology, and development of an optimal...
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