A quantitative assay for measuring the induction of mutations in human peripheral blood T-lymphocytes.

doi:10.1016/0027-5107(90)90047-8

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Article

A quantitative assay for measuring the induction of mutations in human peripheral blood T-lymphocytes.

Mutation research - 1 May 1990

Norimura T, Maher V M, McCormick J J

Abstract excerpt

We optimized conditions for propagating freshly-isolated human peripheral blood T-lymphocytes and cells that had been stored in liquid nitrogen on Day 5 post-isolation, exposing them to mutagens in exponential growth, and measuring the cytotoxicity of the agent from the loss of colony-forming abi...

Topics

7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Cell Division
Cells, Cultured
Drug Resistance
Ethylnitrosourea
Freezing
Humans
Interleukin-2
Mutagenicity Tests
Mutation
T-Lymphocytes
Thioguanine

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