Article
Exchangeable gene trap using the Cre/mutated lox system.
Cellular and molecular biology (Noisy-le-Grand, France) - 1 Jul 1999
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K
Abstract excerpt
The gene trap technique is a powerful approach for characterizing and mutating genes involved in mouse development. However, one shortcoming of gene trapping is the relative inability to induce subtle mutations. This problem can be overcome by introducing a knock-in system into the gene trap strategy. Here, we have constructed a new gene trap vector, pU-Hachi, employing the Cre-mutated lox system (Araki et al.,...
Topics
- Animals
- Clone Cells
- Genetic Techniques
- Genetic Vectors
- Green Fluorescent Proteins
- Integrases
- Luminescent Proteins
- Mice
- Mutagenesis
- Mutation
- Plasmids
- Stem Cells
- Viral Proteins
- beta-Galactosidase