Article
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Genes & development - 1 Sept 1991
Friedrich G, Soriano P
Abstract excerpt
A general strategy for selecting insertion mutations in mice has been devised. Constructs lacking a promoter and including a beta-galactosidase gene, or a reporter gene encoding a protein with both beta-galactosidase and neomycin phosphotransferase activity, were designed so that activation of the reporter gene depends on its insertion within an active transcription unit. Such insertion events create a mutation...
Topics
- Animals
- Blotting, Northern
- Blotting, Southern
- Cell Line
- Cloning, Molecular
- Fetus
- Genetic Vectors
- Germ Cells
- Kanamycin Kinase
- Mice
- Mice, Inbred C57BL
- Mice, Inbred CBA