Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates.

Abstract excerpt

BACKGROUND: Conditional knockout mice are a useful tool to study the function of gene products in a tissue-specific or inducible manner. Classical approaches to generate targeting vectors for conditional alleles are often limited by the availability of suitable restriction sites. Furthermore, plasmid-based targeting vectors can only cover a few kB of DNA which precludes the generation of targeting vectors where...

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