Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutant loxP vectors for selectable marker recycle and conditional knock-outs.

BMC biotechnology - 1 Jan 2001

Arakawa H, Lodygin D, Buerstedde J M

Abstract excerpt

BACKGROUND: Gene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function. The chicken B cell line DT40 has been widely used as a model for gene knock-outs due to its high targeted integration activity. Disruption of multiple genes and complementation of the phenotypes is, however, restricted by the number of available selectable marker genes. It is...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Attachment Sites, Microbiological
Share to Chickens
Share to Gene Deletion

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Vectors

Share to Integrases

Share to Mutagenesis, Insertional

Share to Recombination, Genetic

Share to Viral Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 11591226
DOI: 10.1186/1472-6750-1-7
PMCID: PMC57747

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutant loxP vectors for selectable marker recycle and conditional knock-outs.PMID 11591226Read ↗

Overview of gene targeting by homologous recombination.Semantic proximity Read ↗

A highly efficient recombineering-based method for generating conditional knockout mutations.Semantic proximity Read ↗

Knock-in approaches.Semantic proximity Read ↗

Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates.Semantic proximity Read ↗

Gene targeting in the mouse.Semantic proximity Read ↗

The DT40 web site: sampling and connecting the genes of a B cell line.Semantic proximity Read ↗

A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond.Semantic proximity Read ↗

Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.Semantic proximity Read ↗

LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting.Semantic proximity Read ↗

Production of a heterozygous mutant cell line by homologous recombination (single knockout).Semantic proximity Read ↗

Production of a heterozygous mutant cell line by homologous recombination (single knockout).Semantic proximity Read ↗

Gene targeting in mice: a review.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.