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Article

A highly efficient recombineering-based method for generating conditional knockout mutations.

Genome research - 1 Mar 2003

Liu Pentao, Jenkins Nancy A, Copeland Neal G

Abstract excerpt

Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombineering, has many different uses for functional genomic studies. Here we describe a new recombineering-based method for...

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Identifiers

PMID: 12618378
DOI: 10.1101/gr.749203
PMCID: PMC430283

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