Article
Amber (UAG) suppressors affected in UGA/UAA-specific polypeptide release factor 2 of bacteria: genetic prediction of initial binding to ribosome preceding stop codon recognition.
Genes to cells : devoted to molecular & cellular mechanisms - 1 May 1999
Yoshimura K, Ito K, Nakamura Y
Abstract excerpt
BACKGROUND: Prokaryotic translational release factors, RF1 and RF2, catalyse protein release at UAG/UAA and UGA/UAA stop codons, respectively. Mutations in RF1 and RF2 are known to cause non-sense suppression for UAG (amber) and UGA (opal) codons, respectively, and they do not exert a reciprocal ('cross') suppression phenotype. We aimed to isolate RF mutants of such cross-suppression activity, which we designated...
Topics
- Alleles
- Amino Acid Sequence
- Amino Acid Substitution
- Codon, Terminator
- DNA Mutational Analysis
- Molecular Sequence Data
- Mutation
- Peptide Termination Factors
- Phenotype
- Plasmids
- Recombinant Fusion Proteins
- Salmonella typhimurium
- Sequence Deletion