Article
Suppression of temperature-sensitive defects of polypeptide release factors RF-1 and RF-2 by mutations or by an excess of RF-3 in Escherichia coli.
Journal of molecular biology - 17 May 1996
Matsumura K, Ito K, Kawazu Y, Mikuni O, Nakamura Y
Abstract excerpt
The termination of protein synthesis in bacteria requires two codon-specific polypeptide-release factors, RF-1 and RF-2. A third factor, RF-3, stimulates the RF-1 and RF-2 activities in vitro. To clarify the in vivo role of RF-3 for the RF-2 dependent termination, we isolated and characterized su...
Topics
- Base Sequence
- Codon, Terminator
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Peptide Chain Termination, Translational
- Peptide Termination Factors
- Promoter Regions, Genetic
- Recombinant Proteins
- Suppression, Genetic
- Temperature