Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli.

doi:10.1016/0300-9084(91)90185-4

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Article

Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli.

Biochimie - 1 Dec 1991

Mikuni O, Kawakami K, Nakamura Y

Abstract excerpt

Mutations in the prfB gene which encodes peptide-chain-release factor 2 of Escherichia coli were defined by DNA sequence analysis. prfB1 and prfB3 substitute lysine and asparagine for glutamate and aspartate at amino acid positions 89 and 143, respectively. Temperature-sensitive mutations, prfB2...

Topics

Amino Acid Sequence
Asparagine
Blotting, Southern
Codon
Escherichia coli
Lysine
Molecular Sequence Data
Mutation
Peptide Chain Termination, Translational
Peptide Termination Factors
Protein Biosynthesis
Suppression, Genetic

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