Article
Ribosomal protein L11 mutations in two functional domains equally affect release factors 1 and 2 activity.
Molecular microbiology - 1 Apr 2006
Sato Hanae, Ito Koichi, Nakamura Yoshikazu
Abstract excerpt
Bacterial release factors (RFs) 1 and 2 catalyse translation termination at UAG/UAA and UGA/UAA stop codons respectively. It has been shown that limiting the amount of ribosomal protein L11 affects translation termination at UAG and UGA differently. To understand the functional interplay between L11 and RF1/RF2, we isolated 21 distinct mutations in L11 as suppressors of either temperature-sensitive (ts) RF1/RF2...
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